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Reasons for false-positive, false-negative, and indeterminate results in assays for the detection of antibodies against HIV. (Table.) (Accessed March 10, 2006, at http://www.hivguidelines.org/public_html/a-tests/a-tests-tbl1.htm.)
Chan DC , Fass D , Berger JM , Kim PS . Core Structure of gp41 from the HIV envelope glycoprotein. Cell 1997 ; 89 : 263 - 273
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why does DR HHH say the ELISA is good for CRFs then in some posts he questions the tests reliability of detection of antibodies associated with CRF's?
I get that ELISA and EIA will detect all all subtypes to include CRF's, but is it reliable and conclusive at 12 weeks? I am focused on CRF01_AE.
Does the military use ELISA or EIA?
In short, DUO is a 4th generation ELISA and its effectiveness in detecting the antibodies is the same as that of 3rd generation ELISA tests and reliable approved rapid HIV tests, however its capability to detect early infection is due to its capability to detect P24 antigen which is usually high in early infection and then declines, once the antibodies become greater. Antibodies will then be detectable for the rest of life, except in EXCEPTIONALLY RARE circumstances.
You were protected until the condom failed. High or Low, a risk is a risk. If you test after 4 weeks the antigen part of the duo test would not be conclusive. As with any HIV test the only conclusive test is one taken 3 months post exposure. DUO tests cannot detect HIV 2 any earlier than a straight antibody test.
Y u r nt replyin? plz reply and i will gain my peace of mind....
This will be the last response.
An ELISA is a test format. There are several different "generations" of ELISA tests for HIV, the most recent being the 4th generation tests. ELISA tests test for antibodies, sometimes abbreviated as ab. The combo tests also test for HIV peptides (pieces of protein). All of this however is IRRELEVANT to you as you have no reason for concern at this time. You need to believe your tests. You did not get HIV from the exposure you described.
Yes. The 3rd generation tests for antibodies only while the 4th generation tests for both antibodies and particles of the virus itself.
Hello Doctors,
Excuse my bad english.
First, thank you very much for all what you are doing.
I am a French guy established in Canada for a few months,
6 weeks and 2 days after a low risk sexual exposure, I remained very worried so I performed an HIV test "Elisa combo p24" that came back negative. I know that this result is conclusive according to your previous answers on this forum.
If it is used to measure the viral burden, then the accuracy of detection is very high, so it can detect extremely small amounts of virus in the blood. Why then not use it for conclusive results? The conclusive timeframe for ELISA is 3 months, but before you seroconvert, the virus spreads quickly in your blood, so you are supposed to have large number of infected cells, so PCR should detect that easily!!?!?! Right!?!?!?
s say per chance you were still in the period where you only igM antibodies but not yet igG antibodies, a 2nd generation test would come back negative? It wouldnt pick anything up at all? I've read its like a 2 week window between the 2...
My problem now that I have a very intesresting job offer in KSA. in KSA the government just consider the antibodies test for workers and they do not recognize PCR result.
My question is: Is there any treatment can be done to hide these antibodies in my blood? giving the infromation that the result of ELISA should be less than 1.
There are no tests reliable at 2.5 weeks and rapid tests are ELISA tests. All antibody tests are conclusive at 3 months post exposure.
I was just wondering the average time for the IGg test to show up. I tested at 7.5 weeks HSV-1 positive and HSV-2 Negative. I know it says it can take up to 6 months but it also sounds like most people feel pretty good by a week 12 test? Is this a similar situation as with HIV where the extreme outliers (past 12 weeks to show positive) are generally those with compromised immune systems (chemo, other) or is something else driving this?
A recent medical article stated that the Insti Rapid HIV test can detect the virus nearly 9 days earlier than the Western Blot laboratory test. Furthermore, the test performed rather well against other laboratory based HIV tests. This is due largely to Insti's ability to detect HIV gp41 antibodies, which are produced during early infection. With that said, how accurate is an Insti rapid test at 6.5 weeks after unprotected vaginal sex with a female of unknown status?
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