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Elisa test vs western blot test

Common Questions and Answers about Elisa test vs western blot test

elisa-test

Avatar m tn Western Blot is a confirmation test, done once ELISA antibody test comes positive. WB is not a primary screening test.
Avatar m tn thank you!
Avatar n tn I took an Elisa test and it was positive and I took a Western Blot test and it was negative. The syphilis test was negative. So I am taking another Elisa, Western Blot and PCR test. What are my chances of these tests being positive???
Avatar n tn The doctor told me that my ELISA test was positive and so they sent my blood sample for an Antigen Western Blot test which came back negative. The doctor told me that 99,999% the Elisa test was false because of other factors that might affect the blood but he asked me to retest in one week. Is it possible that I have contacted Hiv? If so, how? I used a condom and it didn't break. I also cannot have Hiv from previus relationships because I tested Hiv negative 9 months ago.
Avatar n tn Is it possible to detect false -ve result in western blot test after performing ELISA test,what is the condition of person who has come with +ve ELISA test but -ve in western blot test.How much accurate are WB test as if ELISA test can have false +ve test then how can some some say that WB test are accurate if come with -ve result.
Avatar n tn The doctor told me that my ELISA test was positive and so they sent my blood sample for an Antigen Western Blot test which came back negative. The doctor told me that 99,999% the Elisa test was false because of other factors that might affect the blood but he asked me to retest in one week. Is it possible that I have contacted Hiv? If so, how? I used a condom and it didn't break. I also cannot have Hiv from previus relationships because I tested Hiv negative 9 months ago.
Avatar m tn We have a patient who was put on suppression, with a positive swab test, and two years later, being on suppression all that time, was not yet positive on the western blot. We pulled her off and in a couple of months was positive on the western blot. Also, the University of Washington now notes on the test results that antiviral therapy can impact the western blot.
Avatar n tn If you test too early after infection, the screening ELISA test that you had done could pick up early infection where the western blot did not. I would advise that before taking the western blot, you wait at least 4 months for an accurate test. If you are negative by western blot for both, and you have waited 4 months and your first ELISA value was between 1.1 and 3.5, then I would consider the western blot the accurate result here.
Avatar n tn They just had their blood work done, and the guy who is supposed to be the bio dad (his sperm will be used to impregnate me) got an indeterminate result on the Elisa test. From all Ive read here, most people get indeterminates on the Western Blot, not the Elisa. Weird? Anyway, his Western Blot was negative, as was his viral load test. Based on his symptoms...fatigue lasting approx 4 weeks, and his location, his drs have decided to treat him for Lyme disease, and do more testing...
Avatar m tn In my laboratory the HerpeSelect ELISA tends to be more accurate than the Western blot. This is not the same test as the Univesity of Washington Western Blot. I had originally thought your Western Blot was from UW but that appears not to be the case. I would revise my assessment to say (again) that your HSV-2 results may be falsely positve. Further, you may or may not have HSV-1.
Avatar m tn I wanted to follow up and see if these new test results would give me any reason to not follow up with a western blot. I would think if I haven't seroconverted by now I never will (is that possible?). Another quick question. A doctor I had 10+ years ago may have put me on an antiviral medication (could have been valtrax). Is there any reason something like that would prevent serocoversion?
Avatar f tn To balance the controversy of PCR test and the lengthy time people have to go through to get ELISA/Rapid + Western Blot test, many believe that it's worth to get HIV DUO test (HIV 1 and 2 antibodies + p24 antigen test), because you can get tested as early as 1 month post-possible exposure.
Avatar n tn If you had a positive test you would have been given a Western Blot or a comparison test to confirm it.
Avatar m tn Of course ELISA test can be indeterminate. No an ELISA test is not that much different than a Rapid test. One takes a machine to process while the other doesn't.
Avatar m tn Wait for your Western blot results and I pray for you that they come back negative. In the meantime, why don't you go for a rapid HIV test with a finger prick? They are not known for giving false positives while the rapid oral tests are slightly more prone to giving false positive results. You can get the results in 20 minutes and then know what to expect while waiting for your WB blot results. I truly wish you the best of luck and you are in my prayers.
Avatar m tn So you guys are telling me that 2 negative Western Blot's mean absolutely nothing without the Elisa? How come if someone has a positive Elisa and a negative Western Blot then it's negative? That obviously means Western Blot is stronger than the Elisa. Sorry if I'm coming out annoying but my question still isn't answered.
Avatar f tn 6 weeks after the last encounter I had an Elisa and a Western blot. The Elisa was reactive, the Western Blot came back indeterminate. The lab person told my provider he didn't think I was positive, but would need to retest in a month. He told my provider it happens sometimes with women who have had kids. Yesterday (4 weeks later at 10 weeks since last encounter) I went to a testing center where I could have anonymous testing done.
Avatar m tn western blot is suppose to be used to confirm hiv infection after an elisa test or rapid test then they usually test on the elisa one more time to make sure it wasnt a false positive if positive then they confirm with the wb
Avatar m tn It's an ELISA test.
Avatar f tn I always take the western blot results over the Herpeselect, yes, because the western blot is a far more complete test. It looks for many proteins associated with the antibody to herpes where the herpeselect only looks for one. You will likely always be positive on the type specific IgG test, yes. I think you have a definite answer with the western blot.
Avatar m tn Both of your HSV 2 results are low positives. The western blot is picking up the HSV 1, and the ELISA is not. The western blot is more sensitive for HSV 1, by quite a bit, than the ELISA, so that is not a surprise. You HSV 1 antibody is making your HSV 2 test look artificially positive, there is some cross reaction going on there. We don't know much more about what causes false positives.
Avatar m tn I have been reading online and have read where some people say there is no difference between the test buts ome say the western blot can pick up positives that the Elisa missed. Further research says the Elisa is looking for 1 peptide that is carrying ant-bodies for HSV2 if you should have it but the western blot is looking at 14 peptides for HSV infection. So my question is does anybody know how many of the 14 peptides in the western blot test could actually help pick up infection ?