Non-alcoholic fatty liver disease (NAFLD) is the most common hepatopathy and is closely associated with metabolic abnormalities. Patients with advanced liver disease exhibit vitamin-D (VD
) deficiency. Our aim was to assess if VD
influences primary hepatic stellate cells (HSC) phenotype and function, and possibly modulates NASH-associated fibrogenesis. Methods: Primary human HSCs and the immortal HSC cell line LX2 were treated with VD2 (10-6M) for 24h.