As I am in a remote area right now, I had to get a HIV
approved for USAID.
I understand these home
kits has the HIV antigen embedded in the capture pad, which then will react if the blood sample is followed by the diluent and an HIV antibody was found in the blood specimen.
According from the manufacturer site, this is what it said:
"The assay starts with a diluent prepared sample applied to the sample well.