Hiv testing elisa western blot

Common Questions and Answers about Hiv testing elisa western blot


Avatar n tn Then he said he was going to have the Western Blot test run. The Western Blot test came back negative. I have looked up the reasons for false positive Elisas online and found a huge list of possible causes. The only thing is I don't seem to fit into any of the categories mentionned to cause the false positive results. The other strange thing is my dad went to donate blood and he tested positive on the Elisa twice and negative on the Western Blot.
Avatar n tn I had blood work done for HIV. The ELISA tested positive, but the Western Blot came back negative. My doctor has only seen this happen twice. He now wants me to come back in for another test and test viral loads. From questions asked by myself as to my risk of transmission, I was at relatively low risk or no risk at all at transmission. Do you think that the second round of tests are needed?
Avatar m tn Why not take the Western Blot immediately instead going thru the Elisa first? Dont you think it's just spending twice for the same test if the Western Blot is always the confirmatory test?
Avatar n tn I went to hiv testing site, and I ask her about this test she ask me do I want western blot also and she took two tubes of bllod for testing, Western Blot is this test better then elisa ???
Avatar f tn Not every MD is very knowledgeable when it comes to HIV and HIV testing. You are HIV-negative, regardless.
Avatar m tn A positive ELISA test is always followed by a Western blot test. A positive Western blot confirms an HIV infection. A negative Western blot test means the ELISA test was a false positive test. The Western blot test can also be unclear, in which case more testing is done. Negative tests do not rule out HIV infection. There is a period of time (called the "window period") between HIV infection and the appearance of anti-HIV antibodies that can be measured.
Avatar m tn Positive Elisa Negative HIV DNA PCR 12 months: Positive Elisa Negative Western Blot What are the chances of not seroconverting up to this point? Do I need additional testing? Do you guys think I'm HIV negative?
Avatar m tn Can I take 2 Western Blot HIV tests and rely on them? The site where I get my HIV testing from doesn't have the Elisa test as an option. I know this test usually comes first. Instead I took 2 Western Blot tests for HIV and they were negative. Because I didn't take the Elisa, can I rely on the Western Blot results? I really need help...
Avatar n tn Also, indeterminate Western blot means nothing. Technically, a Western Blot test detects antibodies to HIV-1, it includes core proteins - p17, p24, p55, envelope glyco-proteins - gp41, gp120, gp160 and polymerase proteins - p31, p51, p66. Western blot test is not recommended as a stand alone alone as it has a 2% rate of false positives. It is advisable to be subordinated with EIA or IFA screening. You did not have any risk for HIV transmission.
Avatar f tn A very small proportion (less than 1%) of persons have antibodies which cross react with some of the proteins contained both in ELISA and Western blot tests for HIV. As a result these persons have positive or equivocal ELISA results and when the Western blot is performed, typically only a single of many possibly reactive protein bands reacts in the test. In contract when a person has antibodies to HIV infection they make antibodies to many bans an many band react.
Avatar m tn In each case, Elisa positive and Western Blot negative. Is further testing warrented if no unsafe sex? For the last five years, my total lymphocyte count has been on the low side. Please advise.
Avatar m tn False positives would mainly only happen in the 4th generation Elisa/Duo tests, and not in the Western blot.
Avatar n tn Is it possible to detect false -ve result in western blot test after performing ELISA test,what is the condition of person who has come with +ve ELISA test but -ve in western blot test.How much accurate are WB test as if ELISA test can have false +ve test then how can some some say that WB test are accurate if come with -ve result.
Avatar n tn How concerned should I be that the RNA will come back positive since the ELISA was positive and the Western Blot was negative, but ELISA and Western Blot was done so early after the possible exposure? How accurate is the ELISA and Western Blot test 18 days from exposure date and is a "false positive" for ELISA any indication of an early syptom of being Positive? I'm very concerned and have been in tears a few times worring about all of this.
Avatar m tn The following day, the doctor tells me it looks like I've contracted HIV, with a positive Elisa test and indeterminate Western blot. Right away, I knew it had to be from that French woman, the only woman I had sex with this past year ! Coincidentally, after more than a month of not hearing from this French woman, she gets back in contact with me and leaves me a message the VERY day I get the bad news.
Avatar f tn I just received notice from my doctor that my Elisa test was reactive and western blot was negative. My boyfriend and I always use condoms, with the exception of maybe 3 times a year or so we slip up. We both tested negative for HIV in August 2012. Anyhow, at the end of May the condom broke once and then again at the end of June. My doctor says I can look at the result as a negative since the western blot was negative and didn't find any bands.
Avatar n tn did you have any chance of exposure during the 6 month period? did you have elisa, western blot and pcr testing?
Avatar f tn To balance the controversy of PCR test and the lengthy time people have to go through to get ELISA/Rapid + Western Blot test, many believe that it's worth to get HIV DUO test (HIV 1 and 2 antibodies + p24 antigen test), because you can get tested as early as 1 month post-possible exposure.
Avatar m tn It was a mistake for you to insist on the Western blot. Had you come to my clinic, we would have refused your request. A negative ELISA, by itself, is 100% proof against HIV if done mor ethan 6-8 weeks after the last possible exposure. WB was not designed as a primary test -- it's only value is in confirming a positive ELISA. And yes, "this is the case in [your] situation" -- since it is the case in everybody's "situation". You can be 100% certain you do not have HIV.
Avatar n tn Is this something that can give me enough peace of mind until my blood work comes back in 7-10 days? Does HIV show up sooner with the Western Blot test? My husband said that the last time he had oral sex with this girl was around June 15th. I still do not understand why his blood work was negative and mine came out the way that it did?? Thanks again!
Avatar f tn The clinic did not do the elisa test only a western blot for hiv-1 which came back with an inderminate result showing non viral bands only. All other antibodies or proteins like p24 showed up negative. Even the doctor was confused and said she was going to have to call the lab because she has never seen that in her 20 year career. I've been a nervous wreck. I've been faithful to one man for years but suspect he's been sleeping around.
Avatar m tn I had an HIV test done today which is 13 weeks post exposure. As I was leaving my county health clinic, I noticed that my paperwork said " western blot" on it. After doing some research, I found out that you aren't supposed to have a western blot test done unless you have already had a positive Elissa test, which I've never had. What will this mean with my test result from the western blot? I'm very nervous as I want my test result to be as accurate as possible.
Avatar n tn Hello, What is a HIV Antibody Test w/Reflex Western Blot? Why would a primary care physician order that test? Isn't a Western Blot Test only used to confirm a positive antibody test? Do they order that test to save time if you had a positive reaction?
Avatar n tn I would be interested to see how a Western Blot and Elisa stack up. There was a question there yesterday about some WB taking longer than others for reasons unknown. The site said something to the effect of 20% or blood takes an additional week or so. I would also like to know why that is? Personally I have never been tested for any STD's but have always practiced safe sex. This site has taught me alot. I plan to use some information to teach my teenage sons more about safe sex.
Avatar n tn (Indeed no diagnostic test is perfect, although modern HIV antibody tests come close, happily.) The Western blot is not 100% sensitive, meaning it misses a few infected people; that is, the non-WB diagnostic tests (e.g., HerpeSelect) actually is a little more sensitive. The main purpose of a WB is to confirm another test that is positive--NOT to diagnose absence of HIV in someone with a positive standard test. Also, WB tends to become positve more slowly than the type-specific ELISA tests.
Avatar f tn The CDC [US Centers for Disease Control, part of the US government, which also doesn't understand what Lyme docs do] still states that Western Blot testing should only be done when the ELISA is first positive. The Website states that IgM results found after the first 4-6 weeks are likely false positive unless 5/10 (that is, half of the] IgG bands appears at that time. These facts are referenced back to findings from 1994-95.
Avatar f tn Dear Terri, I was diagnosed through an IgG blood test from an independent lab as positive for herpes type 2. I am trying to get the Western Blot done. I need confirmation b/c I have had sex less than 20 times in my life, have only been w/two men (though one told me he had 30 partners before me so I probably got it from him), and have had no symptoms. I asked about the numerical range that accompanies the test and the ob/gyn said that it was only positive or negative.
Avatar f tn I was encountered unprotected sex with one well known married women (but I don't know her HIV status) 8 month before now I had fear and anxiety about HIV and I tested many times to clear my fear all my hiv test results are negative but only western blot is indeterminate only one antigen p24 present. My tests and test taken dates are follow 1. 5.3.15 antibody test non reactive 2. 24.3.15 antibody test triline method negative 3. 30.6.15 antibody test non reactive 4. 17.7.