Hiv testing elisa western blot

Common Questions and Answers about Hiv testing elisa western blot


Avatar f tn Western blot test are used to confirm a positive test.
Avatar m tn So you guys are telling me that 2 negative Western Blot's mean absolutely nothing without the Elisa? How come if someone has a positive Elisa and a negative Western Blot then it's negative? That obviously means Western Blot is stronger than the Elisa. Sorry if I'm coming out annoying but my question still isn't answered.
Avatar m tn western blot testing at 8 months resulting indeterminate means negitive.
Avatar n tn Is it possible to detect false -ve result in western blot test after performing ELISA test,what is the condition of person who has come with +ve ELISA test but -ve in western blot test.How much accurate are WB test as if ELISA test can have false +ve test then how can some some say that WB test are accurate if come with -ve result.
Avatar n tn This was using new generation elisa, western blot. If too much testing, and not enough, can anyone reference a credible article with data that show evidence for false negative PCR viral load testing at one month when a individual is has not received post exposure prophylaxsis, is not immunosuppressed, and does not have a coninfection with hepatitis C? My understanding is that most individuals will have detectible viral loads via PCR if done greater than 28 days.
Avatar f tn Why is the western blot used as a confirmation test when an elisa can become positive before it?
Avatar n tn But, wouldn't The Western Blot show 'positive'? If the Elisa was positive and the Western Blot was negative, the Western Blot would be 'definitive', right? I thought the Western Blot was the 'Gold Standard'. Shouldn't I be able to trust it? Why else would 2 doctors, even one trying to protect my pregnant wife order it? Please help, I'm terribly worried.
Avatar n tn The doctor told me that my ELISA test was positive and so they sent my blood sample for an Antigen Western Blot test which came back negative. The doctor told me that 99,999% the Elisa test was false because of other factors that might affect the blood but he asked me to retest in one week. Is it possible that I have contacted Hiv? If so, how? I used a condom and it didn't break. I also cannot have Hiv from previus relationships because I tested Hiv negative 9 months ago.
Avatar f tn Please help. I get tested twice Elisa negative and a p17 appear in western blot .Am I infected?.
Avatar m tn The WB is the royal flush of HIV tests,if it's negative then you don't have HIV--it's that simple.
Avatar m tn A positive ELISA test is always followed by a Western blot test. A positive Western blot confirms an HIV infection. A negative Western blot test means the ELISA test was a false positive test. The Western blot test can also be unclear, in which case more testing is done. Negative tests do not rule out HIV infection. There is a period of time (called the "window period") between HIV infection and the appearance of anti-HIV antibodies that can be measured.
Avatar m tn It was a mistake for you to insist on the Western blot. Had you come to my clinic, we would have refused your request. A negative ELISA, by itself, is 100% proof against HIV if done mor ethan 6-8 weeks after the last possible exposure. WB was not designed as a primary test -- it's only value is in confirming a positive ELISA. And yes, "this is the case in [your] situation" -- since it is the case in everybody's "situation".
Avatar m tn You should be able to have another test performed which looks for different HIV proteins. Does your doctor regularly deal with HIV and HIV testing? If not, perhaps he would be willing to either offer clarification or to send you for a second test or second opinion. Hope this helps.
Avatar n tn hey lily sorry not to burglarize your post but to answer HSci34 yea i went to an infectious disease doctor and she ordered hiv-1 hiv-2 elisa confirm with western blot thats what my lab paper said so i am assuming they did the elisa first then western blot
Avatar m tn I had an HIV test done today which is 13 weeks post exposure. As I was leaving my county health clinic, I noticed that my paperwork said " western blot" on it. After doing some research, I found out that you aren't supposed to have a western blot test done unless you have already had a positive Elissa test, which I've never had. What will this mean with my test result from the western blot? I'm very nervous as I want my test result to be as accurate as possible.
Avatar m tn They did do the western blot. Here are the results: HIV-1 Antibody Test with Reflex to Western Blot Confirmation <1.00 / Non Reactive <1.00 / Non Reactive Negative 04/26/2010 04/27/2010 . I had a direct conversation with the person there and she said they definitely run the western blot wether you have a positive or negative result. Why would the woman not tell me the truth?
Avatar n tn Recnetly went in for my annual visit but this was different because a week later the doctor called me with all lab work which for the most part was excellent but doc informed me of testing possitive to elisa test but negative for western blot. I've been in a committed relationship for ten years with children's father and feel secure.
Avatar f tn The clinic did not do the elisa test only a western blot for hiv-1 which came back with an inderminate result showing non viral bands only. All other antibodies or proteins like p24 showed up negative. Even the doctor was confused and said she was going to have to call the lab because she has never seen that in her 20 year career. I've been a nervous wreck. I've been faithful to one man for years but suspect he's been sleeping around.
Avatar f tn If the ELISA test is positive, a second test called a Western blot is done to confirm the result. The Western blot takes longer to perform and is more expensive than the ELISA test, but it is more precise.
Avatar f tn You require a positive elisa for a western blot to be done
Avatar f tn the dr explained that the western blot looks just for hiv 1. and that the immunoblot looks specifically for hiv 2. I'm so scared. My last exposure was unprotected sex about a year ago. Since that exposure, I had a few rapid tests done. 2 were the unigold rapid test, but they only check for hiv 1. The other one was the oraquick advance. Those 3 were beyond the window period and were non reactive. I do not know what prompted me to get tested again. I almost wish I hadnt.
Avatar m tn At 8 weeks – I went back to my primary care physician and they again did a Western Blot with no ELISA. The results came back indeterminate this time showing a different band, p51 only. At 9 weeks – I went to a specialist, she did a ELISA and RNA PCR. Both came back non reactive and not detected. We are now at week 14 and even after getting my results at week 9 I am very anxious. I keep thinking something still might be wrong. Why did my blood show these random proteins?
Avatar m tn May I know the difference? Why not take the Western Blot immediately instead going thru the Elisa first? Dont you think it's just spending twice for the same test if the Western Blot is always the confirmatory test?
Avatar m tn Can i trust fully in my results, even though the testing center didnt seem to follow what seems to be normal testing procedure in testing for HIV antibodies?
Avatar f tn Because of this a second type of HIV test, the Western Blot, is performed on all samples that test positive for HIV antibodies using the ELISA test. The Western Blot test confirms if a sample is positive or negative. Very rarely, a result comes back ‘indeterminate’, meaning a person has just started to develop antibodies to HIV because it is so close to the time when they were infected.
Avatar n tn Suddenly in Nov doctor called and told me i am testing positive and asked me to do western blot and it came positive. I am really shocked because throughout my life i have never had operations, blood transfusions, drugs etc.We don't drink alcohol or smoke. My husband also had no operations done or blood transfusions or drug. I should state that in February 2008 they did similar blood test for my vitamins which included HIV and it tested negative.