I had blood work done for HIV. The ELISA tested positive, but the WesternBlot came back negative. My doctor has only seen this happen twice. He now wants me to come back in for another test and test viral loads. From questions asked by myself as to my risk of transmission, I was at relatively low risk or no risk at all at transmission. Do you think that the second round of tests are needed?
Then he said he was going to have the WesternBlottest run. The WesternBlottest came back negative. I have looked up the reasons for false positive Elisas online and found a huge list of possible causes. The only thing is I don't seem to fit into any of the categories mentionned to cause the false positive results. The other strange thing is my dad went to donate blood and he tested positive on the Elisa twice and negative on the Western Blot.
So know you can realize that Elisatest just look for the type of antibodies, but the westernblot look for each specifical antibodie so it may take a few weeks more but if the person is trully 100% positive, a western blot on the same day of a positive elisa it would be at least indeterminate .
Here you can learn how hiv test works
I had a HIV 1 proviral DNA test with PCR method at 11,5 days; it was negative. Then I had ELISA & Western blot tests at 6,5 weeks; both were negative too. Should I consider myself as negative or should I need tow wait 12 weeks for another ELISA for confirmation?
A positive ELISA test is always followed by a Westernblottest. A positive Westernblot confirms an HIV infection. A negative Westernblot test means the ELISA test was a false positive test. The Western blot test can also be unclear, in which case more testing is done.
Negative tests do not rule out HIV infection. There is a period of time (called the "window period") between HIV infection and the appearance of anti-HIV antibodies that can be measured.
Negative HIV DNA PCR
What are the chances of not seroconverting up to this point? Do I need additional testing? Do you guys think I'm HIV negative?
Last year, I had another hivtest done. It came back reactive. It was then followed by a westernblot, which was negative. My doctor said that it means i'm negative, but she informed me to follow up with another test. I never followed through with another test, mainly because everything I read online said it was not necessary to. I never had any other exposure. He was the only man I was ever with.
I'm about to get into a serious relationship and before I do so, I want to make sure.
Which one is best out of these two? Does the PCR still count? and if I get a negative on the Western blot without an Elisa am I still negative? Which test all in all are better? Should I go out of my way for an Elisa test? Which one is most accurate, out of all the tests out there?
Also, indeterminate Westernblot means nothing. Technically, a WesternBlottest detects antibodies to HIV-1, it includes core proteins - p17, p24, p55, envelope glyco-proteins - gp41, gp120, gp160 and polymerase proteins - p31, p51, p66.
Western blot test is not recommended as a stand alone alone as it has a 2% rate of false positives. It is advisable to be subordinated with EIA or IFA screening.
You did not have any risk for HIV transmission.
On the H 1/O/2 1/11 test you stated ,1.00 the cut off is 1
On the Western Blot you only have one band P18 which is not even considered. You didn't have a p120/p160 or a p24 or 41 which equals negative.
I've called the clinic twice to ask questions and during the last conversation the nurse practitioner told me that my WesternBlottest did not reveal any HIV band were absent and the one equivocal band would only pose a problem if I have had sex with someone from West-Africa. (Which band would that be) However, she didn't tell me exactly which band was equivocal. If this is the case, why was my Western Blot not ruled as a 'negative' as opposed to 'equivocal'?
I've called the clinic twice to ask questions and during the last conversation the nurse practitioner told me that my WesternBlottest did not reveal any HIV band--all were absent and the one equivocal band would only pose a problem if I have had sex with someone from West-Africa. She didn't tell me which band was equivocal. If this is the case, why was my Western Blot not ruled as a 'negative' as opposed to 'equivocal'?
Is it possible to detect false -ve result in western blot test after performing ELISAtest,what is the condition of person who has come with +ve ELISAtest but -ve in westernblot test.How much accurate are WB test as if ELISA test can have false +ve test then how can some some say that WB test are accurate if come with -ve result.
If an Elisatest comes back positive, then a WB test is done to verify (or refute) it.
Given that pregnancy is one of the things that CAN cause a false positive on Elisa and her WB came back negative, I'd be inclined to agree with Lizzie Lou that she is not infected.
To balance the controversy of PCR test and the lengthy time people have to go through to get ELISA/Rapid + WesternBlottest, many believe that it's worth to get HIV DUO test (HIV 1 and 2 antibodies + p24 antigen test), because you can get tested as early as 1 month post-possible exposure.
(Indeed no diagnostic test is perfect, although modern HIV antibody tests come close, happily.) The Westernblot is not 100% sensitive, meaning it misses a few infected people; that is, the non-WB diagnostic tests (e.g., HerpeSelect) actually is a little more sensitive. The main purpose of a WB is to confirm another test that is positive--NOT to diagnose absence of HIV in someone with a positive standard test. Also, WB tends to become positve more slowly than the type-specific ELISA tests.
Hi doctor, I had n unprotected viginal sex with a lady, dated ard sept 13,
I did n lab elisatest at jan 14, the results came as hiv positive, westen bolt test
incompulsive, then I did 3 quick prick blood test, all 3 results is negative.
My doctor said that my first elisa test was false positive. Im still havin
nightmares.... plz explain more doctor.
western blot is suppose to be used to confirm hiv infection
after an elisatest or rapid test then they usually test on the elisa one more time to make sure it wasnt a false positive if positive then they confirm with the wb
Practice safe sex always, sinc ehep b 14 yrs ago. Have had two HivElisa and Western Blots seven months a part from each other. In each case, Elisa positive and Western Blot negative. Is further testing warrented if no unsafe sex? For the last five years, my total lymphocyte count has been on the low side.
How concerned should I be that the RNA will come back positive since the ELISA was positive and the WesternBlot was negative, but ELISA and WesternBlot was done so early after the possible exposure? How accurate is the ELISA and Western Blot test 18 days from exposure date and is a "false positive" for ELISA any indication of an early syptom of being Positive?
I'm very concerned and have been in tears a few times worring about all of this.
The following day, the doctor tells me it looks like I've contracted HIV, with a positive Elisatest and indeterminate Westernblot. Right away, I knew it had to be from that French woman, the only woman I had sex with this past year !
Coincidentally, after more than a month of not hearing from this French woman, she gets back in contact with me and leaves me a message the VERY day I get the bad news.
I recently been denied life insurance based on the HIV test results thy have received from their lab. The test came back as a positive ELISAtest and an indeterminate WesternBlot with GP160 only detected. I've been married for 15 years and i did have one "safe sex" affair two years ago. Since then, my only partner has been my wife and she recently tested negative when she applied for insurance.
I just received notice from my doctor that my Elisatest was reactive and westernblot was negative. My boyfriend and I always use condoms, with the exception of maybe 3 times a year or so we slip up. We both tested negative for HIV in August 2012. Anyhow, at the end of May the condom broke once and then again at the end of June. My doctor says I can look at the result as a negative since the western blot was negative and didn't find any bands.
after my partner tested positive for hiv , i tested nearly at the 4 months mark by westernblot , which searched for antibodies for gp120,gp160, gp41,p24,p17,p66,p51,p31,p55 and hiv 2 specific band, the result was neagtive and hiv 2 not indicated.
The Content on this Site is presented in a summary fashion, and is intended to be used for educational and entertainment purposes only. It is not intended to be and should not be interpreted as medical advice or a diagnosis of any health or fitness problem, condition or disease; or a recommendation for a specific test, doctor, care provider, procedure, treatment plan, product, or course of action. MedHelp is not a medical or healthcare provider and your use of this Site does not create a doctor / patient relationship. We disclaim all responsibility for the professional qualifications and licensing of, and services provided by, any physician or other health providers posting on or otherwise referred to on this Site and/or any Third Party Site. Never disregard the medical advice of your physician or health professional, or delay in seeking such advice, because of something you read on this Site. We offer this Site AS IS and without any warranties. By using this Site you agree to the following Terms and Conditions. If you think you may have a medical emergency, call your physician or 911 immediately.