potassium, calcium, sodium, magnesium, vitamin
B complex, or vitamins B1, B3, or B6
D. Generally deficiencies of any of the above can cause muscle twitching and spasms (fasciculations) in localized or generalized areas of the body. Get your kidney function, parathyroid gland function and adrenal function tests done because these affect the electrolyte balance in the body. Stress, alcohol, caffeine and fatigue all cause similar symptoms.