Moreover it has to be differentiated from other causes of such symptoms like low amounts of potassium, sodium, calcium, or magnesium in the blood, muscle fatigue or strain from overuse, tendonitis,osteomyelitis,
osteoarthritis, nerve damage- Sciatic nerve pain (radiating pain down the leg)
caused by a slipped disk in the back., Benign tumors or cysts of the femur or tibia and osteosarcoma.
Hope it helps.Take care and pls do keep me posted if you have any additional queries.