elisa test kit principle

Conjugate binds to the Fc portion of antibodies to give pinkish purple dot against a white background But the problem is after that testing, he gave me that tridot testing kit to me along with the test reports, unfortunately I touched the area of that testing kit membrane (with immobilized HIV antigens) which was soaked with my blood sample and testing solutions, I touched it with my finger and immediately kept that finger inside my nose and eyes …..

You can trust this test. They call this as SPOT ELISA kit.

Thank u for the reply Mike but what about the period from exposure for the conclusive results by that Test?

t be reliable at all nor would any other test. 3 months dude is when you need to take your test and get a conclusive test result. If you want to do a Home Access test. Then do it at three months send it in and your results will be conclusive.

Hi Teak and all seniors, kindly please answer my simple question, a million thanks to u all.. i tested negative by using a home test kit 14 weeks after possible exposure, bought from this webpage http://www.****.com.my/, do i need to go for ELISA / Western block test for conformation? Thanks Thanks..

This may not be the best place to ask this t I wondered if anyone knew this one. When a lab is testing your blood for HIV. Do they use the same Elisa assay kit (tray) to test multiple people at the same time? Or is it 1 kit to 1 person?

Does Home access kit use Elisa? I will ask doctor to do West blot.

line at T area. hardly visible. (Home Test). facts is that ACON Rapid test kit is not approved in USA. Heard about it.. 2month after Results are Test Value: 0.022 ; Cut off Value: 0.300 ; Test Result: Negative My few Questions: 1) What result I need to consider as a final result. 2) Why this ACON test kit showed me a very very faint line. 3) Do u have any info regardin this ACON test kit are they approved by FDA etc.

hi i recently ordered a hiv test kit over the internet from a company called Labpro it came to the uk via malysia, it was a elisa test kit and gave a negative result, how confident should i be of this result?

Here is my story 6 months back(in sep 2010) i had sex with a prostitute but unfortunately my condom got ruptured and i got tested 3 months post exposure and now 6 months post exposure and test results are negative(test was performed using 3rd generation elisa kit) and all my symptoms are muscle pains.still i feel these muscle pains especially at the lowe part of my body (both thighs and calf's). Here are my test results 3 months test:0.32(i.e negative) 6 months test:0.50(i.

Absolutely there is a need to confirm both of these values. I would suggest the western blot. You can obtain a test kit from the University of Washington and have your health care provider draw and send the blood or my clinic can arrange testing for you if you become a phone patient of ours. There is about a 40% chance that the HSV 2 could be a false positive.

The Herpes Simplex IgG test is performed using an ELISA (Enzyme Linked Immunosorbent Assay) test kit manufactured by Euroimmun Diagnostics (Germany). This ELISA test provides a quantitative in vitro assay for human antibodies of the IgG class against HSV 1/2 (group specific) viruses in serum or plasma.

m 100% clear after my 5 months exposure, as you remember 1 week ago I red the kit wrongly after 3 hours of the test ( it showed a faint line in the T area), but today I did another test it did come as follow: The C line was: strong colure The T line was: No colure at all but their was a small colure dot Instead of Strong/faint colure line !!! The nurse was surprise because she said if there is something it should be as a line but not small dot.

3) I took a look at the test kit for the HIV rapid test and the test kit itself looks very simple to me, akin to a pregnancy test kit. Is there any reason that the test kit may give false negative results? How reliable is the test kit itself, assuming that it has not expired?

Positive for HSV-2, negative for others ( HIV, HSV1, Hepatitis, Syphilis, Chlamydia, Gonorrhea ). The herpes test kit used was HerpeSelect ELISA for HSV-1 and HSV-2. Specifically, the HerpeSelect 1 ELISA result was 0.35 and the HerpeSelect 2 ELISA result was 8.79 I repeated the tests in September 2009. A different provider who also used HerpeSelect ELISA. Same results as the testing in May - postive for HSV-2, negative for all others.

Just did the test for me so quick and he gave me the kit when the C line showed dark red, and he told me it is negative I put the kit in pocket and then I took the test kit to my car, the kit was wet because of the blood and to dry it I put it under the sun in the care ( it is very sunny in my country) after 1 hour I looked at the kit again and it did show a faint line in the T .

I'm very worried about the fact that I used an unapproved DIY test kit a couple of months back.. I tested again at 11 weeks after i took that test with a proper test in a lab and the result was negative too but I'm concerned that this home test kit may have been dangerous. Would it be possible to contract any kind of blood to blood illness from a test kit like this? It looked fairly clean but I have no idea where it came from really (apart from China) and that's what concerns me.

Could anyone tell me how accurate an Oraquick oral swab test will be at six weeks? I am well aware that three months is conclusive. I just want to know if a six week test will give me some peace of mind while I wait for three months to pass. Thank you.

Elisa is really good at hsv2 (97~%) but not as good for hsv1 (90-91%) after 4 mobths have passed. After 16 weeks WB is superior to Elisa. Elisa is slightly better than biokit. No herpes doesnt cause any dead cells or ling term effects unless there was a bacterail infection additional to the viral lesion but his is very rare and would not go unnoticed.4 I cant say but everyone has little white bumps, ireegularities, ingrown hairs etc.

i ordered at home test throught Atfirst Diagnostics. it came with 2 test. it was the HIV-3L test kit. i tested negative on march 3 and negative on april 17. am i in the clear?

Ok listen my infectious diseases consultant orderd me a lyme blood test im in the UK what they do here is if the ELISA test comes positive they carry out a WESTERN BLOT now after speaking to the guys at the bloodtest unit he kinda gave it away that a westernblot was carried out so this means that the elisa came positive right?

Elisa test kit principle

Common Questions and Answers about Elisa test kit principle

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